| P388 |
IC50 |
= |
0.5 |
ug.mL-1 |
Cytotoxicity against P388 mouse leukemia cells |
CHEMBL1125332 |
| Adenosylhomocysteinase |
Ki |
|
|
nM |
Inhibitory activity against bovine liver S-adenosyl-L-homocysteine hydrolase (AdoHcy); reversibleinhibitor |
CHEMBL1126037 |
| Adenosylhomocysteinase |
k2 |
|
|
|
Kinetic constant against bovine liver S-adenosyl-L-homocysteine hydrolase (AdoHcy); reversibleinhibitor |
CHEMBL1126037 |
| P388 |
Inhibition |
= |
100.0 |
% |
In vitro inhibition against P-388 mouse leukemia cells using growth inhibition assay at the concentration of 100 ug/mL |
CHEMBL1125060 |
| P388 |
Inhibition |
= |
100.0 |
% |
In vitro inhibition against P-388 mouse leukemia cells using growth inhibition assay at the concentration of 50 ug/mL |
CHEMBL1125060 |
| P388 |
Inhibition |
= |
100.0 |
% |
In vitro inhibition against P-388 mouse leukemia cells using growth inhibition assay at the concentration of 25 ug/mL |
CHEMBL1125060 |
| P388 |
Inhibition |
= |
100.0 |
% |
In vitro inhibition against P-388 mouse leukemia cells using growth inhibition assay at the concentration of 12.5 ug/mL |
CHEMBL1125060 |
| P388 |
Inhibition |
= |
100.0 |
% |
In vitro inhibition against P-388 mouse leukemia cells using growth inhibition assay at the concentration of 6.25 ug/mL |
CHEMBL1125060 |
| Adenosylhomocysteinase |
Inhibition |
= |
99.0 |
% |
Inhibition of bovine liver S-adenosyl-homocysteine hydrolase at 0.2 mM |
CHEMBL1122975 |
| L929 |
Inhibition |
= |
65.0 |
% |
Percentage inhibition of plaque formation, after 72 hr of incubation in virus plaque assay, prepared from monolayer cultures of mouse L 929 cells |
CHEMBL1122975 |
| L cells |
Control |
= |
81.0 |
% |
Percentage control of incorporation of [3H]thymidine into DNA of uninfected L-cell cultures after 36h exposure |
CHEMBL1122975 |
| L cells |
Control |
= |
134.0 |
% |
Percentage control of incorporation of [3H]thymidine into DNA of uninfected L-cell cultures after 72 hr exposure |
CHEMBL1122975 |
| Adenosylhomocysteinase |
Ki |
= |
110.0 |
nM |
Binding affinity against S-adenosyl-homocysteine hydrolase |
CHEMBL1122975 |
| Adenosine deaminase |
Ki |
= |
9900.0 |
nM |
Compound was evaluated for competitive inhibition of adenosine deaminase and expressed as Ki |
CHEMBL1122622 |
| Mus musculus |
ED50 |
= |
67.0 |
uM |
Effective dose by 50% was evaluated |
CHEMBL1122622 |
| Mus musculus |
Activity |
|
|
|
Compound was tested for antileukemic activity in P388 leukemia mice when administered a dose of 100 mg/kg/day; IA means inactive |
CHEMBL1122622 |
| Adenosine A1 receptor |
Ki |
= |
6260.0 |
nM |
Displacement of [3H]R-PIA from rat brain membrane Adenosine A1 receptor |
CHEMBL1133730 |
| Adenosine A2a receptor |
Ki |
= |
2150.0 |
nM |
Displacement of specific [3H]-CGS- 21680 binding to adenosine A2A receptor in rat striatal membranes |
CHEMBL1133730 |
| Adenosine A2b receptor |
Ki |
= |
47300.0 |
nM |
Displacement of [3H]- ZM-241385 from human adenosine A2B receptor expressed in HEK cells |
CHEMBL1133730 |
| Adenosine A3 receptor |
Ki |
= |
20000.0 |
nM |
Displacement of [125I]- AB-MECA from rat adenosine A3 receptor expressed in CHO cells |
CHEMBL1133730 |
| Adenosine receptors; A1 & A3 |
Ratio |
= |
0.31 |
|
Relative binding affinity for Adenosine A1 and A3 receptors |
CHEMBL1133730 |
| Adenosine A1 receptor |
Displacement |
= |
3.0 |
% |
Binding affinity determined on Adenosine A1 receptor in rat brain membranes by measuring displacement of specific [3H]PIA as radioligand. |
CHEMBL1128285 |
| Adenosine A2a receptor |
Displacement |
= |
0.0 |
% |
Binding affinity determined on Adenosine A2A receptor in rat striatal membranes by measuring displacement of specific [3H]-CGS- 21680 as radioligand. |
CHEMBL1128285 |
| Adenosine A3 receptor |
Ki |
= |
200000.0 |
nM |
Binding affinity determined by displacement of specific binding of [125I]N-(4-amino-3-iodophenethyl)-adenosine in membranes of CHO cells stably transfected with the rat adenosine A3 receptor |
CHEMBL1128285 |
| Adenosylhomocysteinase |
IC50 |
= |
4850.0 |
nM |
Inhibition of human SAHH |
CHEMBL1142404 |
| Adenosylhomocysteinase |
IC50 |
= |
57000.0 |
nM |
Inhibition of Plasmodium falciparum SAHH |
CHEMBL1142404 |
| Unchecked |
Ratio IC50 |
= |
0.085 |
|
Selectivity ratio of IC50 for human SAHH to IC50 for Plasmodium falciparum SAHH |
CHEMBL1142404 |
| ADMET |
Activity |
= |
22.4 |
% |
Metabolic stability in presence of calf intestinal adenosine deaminase assessed as compound remaining after 15 mins |
CHEMBL1142404 |
| Trichomonas vaginalis |
IC50 |
= |
10000.0 |
nM |
Antitrypanosomal activity against Trichomonas vaginalis T1 |
CHEMBL1287745 |
| Trichomonas vaginalis |
GI |
= |
95.0 |
% |
Antiparasitic activity against Trichomonas vaginalis T1 at 10 uL after 24 hrs by hemocytometric analysis |
CHEMBL1287745 |
| Trichomonas vaginalis |
IC50 |
= |
3.0 |
ug.mL-1 |
Antitrypanosomal activity against Trichomonas vaginalis T1 |
CHEMBL1287745 |
| Adenosylhomocysteinase |
IC50 |
= |
303.0 |
nM |
Inhibition of recombinant human AHCY using SAH as substrate assessed as formation of homocysteine after 10 mins |
CHEMBL3286270 |
| Adenosylhomocysteinase |
IC50 |
= |
1320.0 |
nM |
Inhibition of human placental SAH hydrolase expressed in Escherichia coli JM109 using SAH as substrate preincubated for 10 mins followed by SAH addition and measured after 20 mins by DNTB reagent-based spectrophotometric method |
CHEMBL4359835 |
| Unchecked |
EC50 |
> |
50000.0 |
nM |
Inhibition of RNA-dependent RNA polymerase in MERS-CoV EMC/2012 infected in African green monkey Vero cells assessed as reduction in virus-induced cytopathic effect measured after 72 hrs post-infection by MTS assay |
CHEMBL4359835 |
| Vero |
CC50 |
= |
2000.0 |
nM |
Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability measured after 72 hrs by MTS assay |
CHEMBL4359835 |
| Unchecked |
EC50 |
> |
50000.0 |
nM |
Inhibition of RNA-dependent RNA polymerase in SARS-CoV Frankfurt1 infected in African green monkey Vero E6 cells assessed as reduction in virus-induced cytopathic effect measured after 72 hrs post-infection by MTS assay |
CHEMBL4359835 |
| Vero C1008 |
CC50 |
>= |
2400.0 |
nM |
Cytotoxicity against African green monkey Vero E6 cells assessed as reduction in cell viability measured after 72 hrs by MTS assay |
CHEMBL4359835 |
| NS5 |
EC50 |
= |
640.0 |
nM |
Inhibition of RNA-dependent RNA polymerase in ZIKV SL0612 infected in African green monkey Vero cells assessed as reduction in virus-induced cytopathic effect measured after 96 hrs post-infection by MTS assay |
CHEMBL4359835 |
| Vero |
CC50 |
= |
2400.0 |
nM |
Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability measured after 96 hrs by MTS assay |
CHEMBL4359835 |
| Unchecked |
EC50 |
= |
800.0 |
nM |
Inhibition of RNA-dependent RNA polymerase in CHIKV LS3 infected in African green monkey Vero E6 cells assessed as reduction in virus-induced cytopathic effect measured after 96 hrs post-infection by MTS assay |
CHEMBL4359835 |
| Unchecked |
Ratio CC50/EC50 |
= |
3.8 |
|
Selectivity index, ratio of CC50 for African green monkey Vero cells to EC50 for inhibition of RNA-dependent RNA polymerase in ZIKV SL0612 infected in African green monkey Vero cells assessed as reduction in virus-induced cytopathic effect |
CHEMBL4359835 |
| Unchecked |
Ratio CC50/EC50 |
= |
7.9 |
|
Selectivity index, ratio of CC50 for African green monkey Vero E6 cells to EC50 for inhibition of RNA-dependent RNA polymerase in CHIKV LS3 infected in African green monkey Vero E6 cells assessed as reduction in virus-induced cytopathic effect |
CHEMBL4359835 |
| Hepatitis B virus |
IC50 |
> |
3000.0 |
nM |
Antiviral activity against Hepatitis B virus infected in human HepG2.2.15.7 assessed as reduction in HBV DNA level incubated for 9 days media replaced every 3 days once with test compound by RT-PCR analysis |
CHEMBL4699415 |
| Unchecked |
CC50 |
> |
3000.0 |
nM |
Cytotoxicity against human HepG2.2.15.7 cells assessed as reduction in cell viability |
CHEMBL4699415 |
