| Unchecked |
K 0.5 |
|
|
|
Tested for stimulation of production of inositol phosphate in turkey erythrocyte membrane; Not active |
CHEMBL1127060 |
| Cavia porcellus |
Relative activity |
|
|
|
Tested for relaxation of carbachol-contracted guinea pig taenia coli at 37 degree C; Significantly less potent than ATP |
CHEMBL1127060 |
| Oryctolagus cuniculus |
Relative activity |
= |
4.8 |
|
Tested for relaxation of carbachol-contracted rabbit aorta at 37 degree C; Potency equal to 2MeSATP |
CHEMBL1127060 |
| Oryctolagus cuniculus |
Relative activity |
= |
5.0 |
|
Tested for relaxation of carbachol-contracted rabbit mesenteric artery at 37 degree C; Less potent or equal to 2MeSATP |
CHEMBL1127060 |
| Oryctolagus cuniculus |
Relative activity |
|
|
|
Tested for contraction of rabbit saphenous artery produced by 1 uM alpha,beta-MeATP at 37 degree C; Not active |
CHEMBL1127060 |
| Cavia porcellus |
Relative activity |
|
|
|
Tested for contraction of guinea pig vas deferens at 37 degree C; Not active |
CHEMBL1127060 |
| Cavia porcellus |
Relative activity |
|
|
|
Tested for contraction of guinea pig isolated urinary bladder detrusor muscle at 37 degree C; Not active |
CHEMBL1127060 |
| Human immunodeficiency virus type 1 protease |
k_on |
= |
4430000.0 |
M-1.s-1 |
Association rate constant for the interaction between inhibitor and HIV-1 protease |
CHEMBL1135671 |
| Human immunodeficiency virus type 1 protease |
k_off |
= |
0.00488 |
s-1 |
Dissociation rate constant for the interaction between inhibitor and HIV-1 protease |
CHEMBL1135671 |
| Human immunodeficiency virus type 1 protease |
Kd |
= |
1.13 |
nM |
Equilibrium constant for the interaction between inhibitor and HIV-1 Protease |
CHEMBL1135671 |
| Human immunodeficiency virus type 1 protease |
Ki |
= |
0.23 |
nM |
Inhibition constant against HIV-1 Protease |
CHEMBL1135671 |
| Unchecked |
Radioactivity |
= |
5.3 |
% |
Percent of compound radioactivity ([3H]-Ap4A Degradation), determined in the incubation medium of INS-1 cell membranes |
CHEMBL1144917 |
| Unchecked |
Radioactivity |
= |
0.8 |
% |
Percent of compound radioactivity ([3H]-Ap4A Degradation), in presence of added Ap4A, in the incubation medium of INS-1 cell membranes |
CHEMBL1144917 |
| Rattus norvegicus |
Radioactivity |
= |
2.4 |
% |
Percent of compound radioactivity ([3H]-Ap4A Degradation) in heparinized rat plasma (saline) |
CHEMBL1144917 |
| Rattus norvegicus |
Radioactivity |
= |
1.98 |
% |
Percent of compound radioactivity ([3H]-Ap4A Degradation), in presence of added Ap(s)pCH2pp(s)A, in heparinized rat plasma |
CHEMBL1144917 |
| Rattus norvegicus |
Radioactivity |
= |
2.31 |
% |
Percent of compound radioactivity ([3H]-Ap4A Degradation), in presence of added Ap(s)pCHClpp(s)A, in heparinized rat plasma |
CHEMBL1144917 |
| Adenylate kinase 2 |
Km |
= |
90000.0 |
nM |
Concentration for half-maximal velocity for adenylate kinase (AK II) was determined |
CHEMBL1122067 |
| Adenylate kinase 2 |
Vmax |
= |
100.0 |
% |
Inhibitory constant of rat adenylate kinase (AK II) isozymes expressed as relative maximal velocity |
CHEMBL1122067 |
| Adenylate kinase 3 alpha like 1 |
Km |
= |
90000.0 |
nM |
Concentration for half-maximal velocity for adenylate kinase (AK III) was determined |
CHEMBL1122067 |
| Adenylate kinase 3 alpha like 1 |
Vmax |
= |
100.0 |
% |
Inhibitory constant of rat adenylate kinase (AK III) isozymes expressed as relative maximal velocity |
CHEMBL1122067 |
| Unchecked |
Km |
= |
610000.0 |
nM |
Concentration for half-maximal velocity for adenylate kinase (AK) was determined |
CHEMBL1122067 |
| Unchecked |
Vmax |
= |
100.0 |
% |
Inhibitory constant of rat adenylate kinase (AK) isozymes expressed as relative maximal velocity |
CHEMBL1122067 |
| Phosphatidylinositol 4-kinase, PI4K |
Inhibition |
= |
31.3 |
% |
Inhibition of Phosphatidylinositol 4-kinase at the ATP binding site at 500 uM |
CHEMBL1124860 |
| Unchecked |
Km |
= |
240000.0 |
nM |
Michaelis rate constant is determined by the Lineweaver and Burk method against PRPP synthetase |
CHEMBL1134666 |
| Unchecked |
Relative Vmax |
= |
14.0 |
|
Maximum velocity constant of the rate of phosphorylation was determined against PRPP synthetase |
CHEMBL1134666 |
| Fructose-1,6-bisphosphatase |
IC50 |
= |
800.0 |
nM |
Concentration required to inhibit the human liver recombinant fructose-1,6-bisphosphatase. |
CHEMBL1134043 |
| P2Y purinoceptor 1 |
NE |
|
|
|
Measure of Agonist Potency at turkey Purinoceptor P2Y1 stably expressed in 131N1 astrocytoma cell; NE means no effect at 100 uM |
CHEMBL1135398 |
| P2Y purinoceptor 1 |
NE |
|
|
|
Measure of Agonist Potency at turkey Purinoceptor P2Y1 stably expressed in 131N1 astrocytoma cell; NE means no effect at 100 uM |
CHEMBL1135398 |
| P2Y purinoceptor 1 |
EC50 |
= |
3500.0 |
nM |
Measure of Agonist Potency at turkey Purinoceptor P2Y1 stably expressed in 131N1 astrocytoma cell |
CHEMBL1135398 |
| Purinergic receptor P2Y1 |
EC50 |
= |
7200.0 |
nM |
Measure of Agonist Potency at human P2Y purinoceptor 1 (hP2Y1) stably expressed in 131N1 astrocytoma cell |
CHEMBL1135398 |
| Purinergic receptor P2Y2 |
EC50 |
= |
3700.0 |
nM |
Measure of Agonist Potency at human P2Y purinoceptor 2 (hP2Y2) stably expressed in 131N1 astrocytoma cell |
CHEMBL1135398 |
| Pyrimidinergic receptor P2Y4 |
NE |
|
|
|
Measure of Agonist Potency at human P2Y purinoceptor 4 (hP2Y4) stably expressed in 131N1 astrocytoma cell; NE means no effect at 100 uM |
CHEMBL1135398 |
| Purinergic receptor P2Y11 |
NE |
|
|
|
Measure of Agonist Potency at human P2Y purinoceptor 11 (hP2Y11) stably expressed in 131N1 astrocytoma cell at 10 uM; NE means no effect at 100 uM |
CHEMBL1135398 |
| P2Y purinoceptor 1 |
EC50 |
= |
2800.0 |
nM |
Measure of Agonist Potency at turkey Purinoceptor P2Y1 stably expressed in 131N1 astrocytoma cell |
CHEMBL1135398 |
| Purinergic receptor P2Y1 |
EC50 |
= |
1500.0 |
nM |
Measure of Agonist Potency at human P2Y purinoceptor 1 (hP2Y1) stably expressed in 131N1 astrocytoma cell |
CHEMBL1135398 |
| Purinergic receptor P2Y2 |
EC50 |
= |
85.0 |
nM |
Measure of Agonist Potency at human P2Y purinoceptor 2 (hP2Y2) stably expressed in 131N1 astrocytoma cell |
CHEMBL1135398 |
| Pyrimidinergic receptor P2Y4 |
EC50 |
|
|
nM |
Measure of Agonist Potency at human P2Y purinoceptor 4 (hP2Y4) stably expressed in 131N1 astrocytoma cell; Antagonist effect |
CHEMBL1135398 |
| Purinergic receptor P2Y11 |
EC50 |
= |
17250.0 |
nM |
Measure of Agonist Potency at human P2Y purinoceptor 11 (hP2Y11) stably expressed in 131N1 astrocytoma cell at 10 uM |
CHEMBL1135398 |
| P2Y purinoceptor 1 |
NE |
|
|
|
Agonist activity by measuring Purinoceptor P2Y1-promoted phospholipase C activity in turkey erythrocyte membranes; No effect as either agonist or antagonist at 10 uM |
CHEMBL1135410 |
| Unchecked |
Activity |
= |
100.0 |
% |
Hydrolysis rate of compound as substrate of purified recombinant rat Ecto-5'-nucleotidase |
CHEMBL1135410 |
| Choline acetylase |
IC50 |
= |
1800000000000.0 |
nM |
Compound was tested for inhibition of choline acetyltransferase isolated from squid head ganglia |
CHEMBL1121773 |
| Vaccinia virus |
MIC50 |
= |
2.0 |
ug.mL-1 |
The antiviral activity was evaluated in primary rabbit kidney(PRK) against Vaccinia virus |
CHEMBL1123099 |
| Human herpesvirus 1 |
MIC50 |
= |
7.0 |
ug.mL-1 |
The antiviral activity was evaluated in primary rabbit kidney(PRK) against Herpes simplex-1 virus |
CHEMBL1123099 |
| Human herpesvirus 2 |
MIC50 |
= |
7.0 |
ug.mL-1 |
The antiviral activity was evaluated in primary rabbit kidney(PRK) against Herpes simplex-2 virus |
CHEMBL1123099 |
| Vesicular stomatitis virus |
MIC50 |
= |
2.0 |
ug.mL-1 |
The antiviral activity was evaluated in primary rabbit kidney(PRK) against Vesicular stomatitis virus |
CHEMBL1123099 |
| Oryctolagus cuniculus |
MIC50 |
= |
20.0 |
ug.mL-1 |
The cytotoxicity was evaluated against primary rabbit kidney(PRK) activity |
CHEMBL1123099 |
| Mammalian orthoreovirus 1 |
MIC50 |
>= |
10.0 |
ug.mL-1 |
The antiviral activity was evaluated in Vero cells against Reo-1 virus |
CHEMBL1123099 |
| Human parainfluenza virus 1 |
MIC50 |
= |
2.0 |
ug.mL-1 |
The antiviral activity was evaluated in Vero cells against Parainfluenza-1 virus |
CHEMBL1123099 |
| Human coxsackievirus B4 |
MIC50 |
>= |
10.0 |
ug.mL-1 |
The antiviral activity was evaluated in Vero cells against Coxsackie B4 virus |
CHEMBL1123099 |
| Sindbis virus |
MIC50 |
> |
10.0 |
ug.mL-1 |
The antiviral activity was evaluated in Vero cells against sindbis virus |
CHEMBL1123099 |
| Semliki forest virus |
MIC50 |
>= |
10.0 |
ug.mL-1 |
The antiviral activity was evaluated in Vero cells against forest virus |
CHEMBL1123099 |
| Measles virus |
MIC50 |
>= |
10.0 |
ug.mL-1 |
The antiviral activity was evaluated in Vero cells against Measles virus |
CHEMBL1123099 |
| Vero |
MIC50 |
= |
10.0 |
ug.mL-1 |
The cytotoxicity was evaluated against Vero cells |
CHEMBL1123099 |
| Vesicular stomatitis virus |
MIC50 |
= |
7.0 |
ug.mL-1 |
The antiviral activity was evaluated in HeLa cells against Vesicular stomatitis virus |
CHEMBL1123099 |
| Human coxsackievirus B4 |
MIC50 |
> |
10.0 |
ug.mL-1 |
The antiviral activity was evaluated in HeLa cells against Coxsackie B4 virus |
CHEMBL1123099 |
| Human poliovirus 1 |
MIC50 |
> |
10.0 |
ug.mL-1 |
The antiviral activity was evaluated in HeLa cells against Polio-1 virus |
CHEMBL1123099 |
| HeLa |
MIC50 |
= |
20.0 |
ug.mL-1 |
The cytotoxicity was evaluated against HeLa cells |
CHEMBL1123099 |
| L1210 |
MIC50 |
= |
3.1 |
ug.mL-1 |
Compound was evaluated for the antitumor activity in L1210 proliferation cells |
CHEMBL1123099 |
| Vaccinia virus |
MIC50 |
> |
400.0 |
ug.mL-1 |
The antiviral activity was evaluated in primary rabbit kidney(PRK) against Vaccinia virus |
CHEMBL1123099 |
| Human herpesvirus 1 |
MIC50 |
= |
200.0 |
ug.mL-1 |
The antiviral activity was evaluated in primary rabbit kidney(PRK) against Herpes simplex-1 virus |
CHEMBL1123099 |
| Human herpesvirus 2 |
MIC50 |
>= |
400.0 |
ug.mL-1 |
The antiviral activity was evaluated in primary rabbit kidney(PRK) against Herpes simplex-2 virus |
CHEMBL1123099 |
| Vesicular stomatitis virus |
MIC50 |
> |
400.0 |
ug.mL-1 |
The antiviral activity was evaluated in primary rabbit kidney(PRK) against Vesicular stomatitis virus |
CHEMBL1123099 |
| Oryctolagus cuniculus |
MIC50 |
> |
400.0 |
ug.mL-1 |
The cytotoxicity was evaluated against primary rabbit kidney(PRK) activity |
CHEMBL1123099 |
| Mammalian orthoreovirus 1 |
MIC50 |
> |
200.0 |
ug.mL-1 |
The antiviral activity was evaluated in Vero cells against Reo-1 virus |
CHEMBL1123099 |
| Human parainfluenza virus 1 |
MIC50 |
> |
200.0 |
ug.mL-1 |
The antiviral activity was evaluated in Vero cells against Parainfluenza-1 virus |
CHEMBL1123099 |
| Human coxsackievirus B4 |
MIC50 |
> |
200.0 |
ug.mL-1 |
The antiviral activity was evaluated in Vero cells against Coxsackie B4 virus |
CHEMBL1123099 |
| Sindbis virus |
MIC50 |
> |
200.0 |
ug.mL-1 |
The antiviral activity was evaluated in Vero cells against sindbis virus |
CHEMBL1123099 |
| Semliki forest virus |
MIC50 |
> |
200.0 |
ug.mL-1 |
The antiviral activity was evaluated in Vero cells against forest virus |
CHEMBL1123099 |
| Measles virus |
MIC50 |
> |
200.0 |
ug.mL-1 |
The antiviral activity was evaluated in Vero cells against Measles virus |
CHEMBL1123099 |
| Vero |
MIC50 |
> |
400.0 |
ug.mL-1 |
The cytotoxicity was evaluated against Vero cells |
CHEMBL1123099 |
| Vesicular stomatitis virus |
MIC50 |
> |
400.0 |
ug.mL-1 |
The antiviral activity was evaluated in HeLa cells against Vesicular stomatitis virus |
CHEMBL1123099 |
| Human coxsackievirus B4 |
MIC50 |
> |
400.0 |
ug.mL-1 |
The antiviral activity was evaluated in HeLa cells against Coxsackie B4 virus |
CHEMBL1123099 |
| Human poliovirus 1 |
MIC50 |
> |
400.0 |
ug.mL-1 |
The antiviral activity was evaluated in HeLa cells against Polio-1 virus |
CHEMBL1123099 |
| HeLa |
MIC50 |
> |
400.0 |
ug.mL-1 |
The cytotoxicity was evaluated against HeLa cells |
CHEMBL1123099 |
| L1210 |
MIC50 |
> |
100.0 |
ug.mL-1 |
Compound was evaluated for the antitumor activity in L1210 proliferation cells |
CHEMBL1123099 |
| Adenylate kinase 2 |
Km |
= |
80000.0 |
nM |
Concentration of compound for half-maximal velocity to Adenylate kinase II |
CHEMBL1122090 |
| Adenylate kinase 2 |
Relative Vmax |
= |
100.0 |
|
Relative Vmax value to rat adenylate kinase II. |
CHEMBL1122090 |
| Adenylate kinase 3 alpha like 1 |
Km |
= |
80000.0 |
nM |
Concentration for half-maximal velocity to Adenylate kinase III |
CHEMBL1122090 |
| Adenylate kinase 3 alpha like 1 |
Relative Vmax |
= |
100.0 |
|
Relative Vmax value to rat adenylate kinase III. |
CHEMBL1122090 |
| Adenylate kinase 1 |
Km |
= |
580000.0 |
nM |
Concentration of compound for half-maximal velocity to Adenylate kinase M isoenzyme |
CHEMBL1122090 |
| Adenylate kinase 1 |
Relative Vmax |
= |
100.0 |
|
Relative Vmax value to Adenylate kinase M isoenzyme (adenylate kinase 1, AK1) |
CHEMBL1122090 |
| Adenylate kinase 2 |
Ki |
= |
80000.0 |
nM |
Inhibitory activity against rat adenylate kinase II was determined in the presence of AMP |
CHEMBL1121949 |
| Adenylate kinase 1 |
Ki |
= |
580000.0 |
nM |
Inhibitory activity against rat Adenylate kinase M isoenzyme in the presence of AMP |
CHEMBL1121949 |
| Inosine-5'-monophosphate dehydrogenase |
Ki |
= |
450000.0 |
nM |
Inhibition of inosine monophosphate dehydrogenase in Escherichia coli |
CHEMBL1121885 |
| Alcohol dehydrogenase |
Ki |
= |
38000.0 |
nM |
Inhibition constant was evaluated against horse liver alcohol dehydrogenase |
CHEMBL1121822 |
| No relevant target |
Kd |
= |
2700.0 |
nM |
Dissociation constant of the compound |
CHEMBL1138674 |
| Ketopantoate reductase |
Kd |
> |
5000000.0 |
nM |
Binding affinity to Escherichia coli KPR |
CHEMBL1145346 |
| Ketopantoate reductase |
Activity |
|
|
|
Inhibition of Escherichia coli KPR at 1 mM |
CHEMBL1145346 |
| Ketopantoate reductase |
Ki |
= |
6300000.0 |
nM |
Inhibition of Escherichia coli KPR |
CHEMBL1145346 |
| 5-methylthioadenosine/S-adenosylhomocysteine deaminase |
Km |
|
|
|
Activity of Thermotoga maritima S-adenosyl-homocysteine deaminase Tm0936 assessed as ammonia production |
CHEMBL1145524 |
| 5-methylthioadenosine/S-adenosylhomocysteine deaminase |
Kcat |
= |
1.0 |
10^-3/s |
Activity of Thermotoga maritima S-adenosyl-homocysteine deaminase Tm0936 assessed as ammonia production |
CHEMBL1145524 |
| 5-methylthioadenosine/S-adenosylhomocysteine deaminase |
Kcat/Km |
|
|
|
Ratio of Kcat to Km for Thermotoga maritima S-adenosyl-homocysteine deaminase Tm0936 assessed as ammonia production |
CHEMBL1145524 |
| Tyrosine-protein kinase SRC |
IC50 |
= |
100.0 |
nM |
Inhibition of p60c-src expressed in chick embryo fibroblast |
CHEMBL1150213 |
| Unchecked |
IC50 |
= |
300.0 |
nM |
Inhibition of Rous sarcoma virus p60 v-src-induced IgG phosphorylation in tumor bearing rabbits by SDS PAGE |
CHEMBL1150213 |
| Fructose-1,6-bisphosphatase |
IC50 |
= |
1000.0 |
nM |
Inhibition of human liver FBPase expressed in Escherichia coli by spectrophotometry |
CHEMBL1138458 |
| Fructose-1,6-bisphosphatase |
IC50 |
= |
1300.0 |
nM |
Inhibition of pig FBPase expressed in Escherichia coli EK1601 by spectrophotometry |
CHEMBL1158422 |
| Unchecked |
Kd |
= |
5400.0 |
nM |
Binding affinity to Mycobacterium tuberculosis adenosine-5'-phosphosulfate reductase assessed as S-sulfocysteine formation at pH 5.5 by single turnover method in absence of thioredoxin |
CHEMBL1154785 |
| Unchecked |
Kd |
= |
5400.0 |
nM |
Binding affinity to Mycobacterium tuberculosis adenosine-5'-phosphosulfate reductase assessed as S-sulfocysteine formation at pH 7.5 by single turnover method in absence of MgCl2 |
CHEMBL1154785 |
| Unchecked |
Kd |
= |
21000.0 |
nM |
Binding affinity to Mycobacterium tuberculosis adenosine-5'-phosphosulfate reductase assessed as S-sulfocysteine formation at pH 7.5 by single turnover method in presence of 0.5 mM MgCl2 |
CHEMBL1154785 |
| Unchecked |
Kd |
= |
31000.0 |
nM |
Binding affinity to Mycobacterium tuberculosis adenosine-5'-phosphosulfate reductase assessed as S-sulfocysteine formation at pH 7.5 by single turnover method in presence of 2 mM MgCl2 |
CHEMBL1154785 |
| Unchecked |
Kd |
= |
5400.0 |
nM |
Displacement of [35S]APS from Mycobacterium tuberculosis adenosine-5'-phosphosulfate reductase by liquid scintillation counting |
CHEMBL1154785 |
| No relevant target |
pKa |
= |
8.1 |
|
Dissociation constant, pKa at pH 6 to 9.5 |
CHEMBL1154785 |
| Unchecked |
Ratio |
= |
5.0 |
|
Ratio of Kd for Mycobacterium tuberculosis adenosine-5'-phosphosulfate reductase in presence of 0.5 mM MgCl2 to Kd Mycobacterium tuberculosis adenosine-5'-phosphosulfate reductase in absence of MgCl2 |
CHEMBL1154785 |
| Unchecked |
Ratio |
= |
5.0 |
|
Ratio of Kd for Mycobacterium tuberculosis adenosine-5'-phosphosulfate reductase in presence of 2 mM MgCl2 to Kd Mycobacterium tuberculosis adenosine-5'-phosphosulfate reductase in absence of MgCl2 |
CHEMBL1154785 |
| 5'-nucleotidase |
Km |
= |
14000.0 |
nM |
Activity at ecto-5'-nucleotidase |
CHEMBL1155272 |
| Alkaline phosphatase, tissue-nonspecific isozyme |
Km |
= |
441000.0 |
nM |
Activity at alkaline phosphatase |
CHEMBL1155272 |
| Fructose-1,6-bisphosphatase |
IC50 |
= |
440.0 |
nM |
Inhibition of FBPase in human liver |
CHEMBL1153201 |
| Fructose-1,6-bisphosphatase |
IC50 |
= |
140.0 |
nM |
Inhibition of FBPase in human liver using fructose-2,6phosphate as a substrate |
CHEMBL1153201 |
| Fructose-1,6-bisphosphatase |
IC50 |
= |
1300.0 |
nM |
Inhibition of pig kidney fructose-1,6-bisphosphatase expressed in Escherichia coli BL21 (DE3) assessed as reduction of NADP+ to NADPH by phosphoglucose isomerase/glucose-6-phosphate dehydrogenase coupled assay |
CHEMBL1157047 |
| Fructose-1,6-bisphosphatase |
IC50 |
= |
9800.0 |
nM |
Inhibition of human liver fructose-1,6-bisphosphatase expressed in Escherichia coli BL21 (DE3) assessed as reduction of NADP+ to NADPH by phosphoglucose isomerase/glucose-6-phosphate dehydrogenase coupled assay |
CHEMBL1157047 |
| Unchecked |
AC50 |
= |
56000.0 |
nM |
Activation of rat liver AMPK assessed as concentration required to induce 50 % activity |
CHEMBL1240408 |
| Survival motor neuron protein |
Potency |
= |
31622.8 |
nM |
PUBCHEM_BIOASSAY: qHTS Assay for Enhancers of SMN2 Splice Variant Expression. (Class of assay: confirmatory) |
CHEMBL1201862 |
| Luciferase |
Potency |
= |
1995.3 |
nM |
PUBCHEM_BIOASSAY: Counterscreen for Luciferase (Kinase-Glo TM) Inhibition. (Class of assay: confirmatory) |
CHEMBL1201862 |
| Unchecked |
EC50 |
= |
1400.0 |
nM |
Activation of rat GPPase |
CHEMBL1687696 |
| Unchecked |
IC50 |
= |
1000.0 |
nM |
Inhibition of rat FBPase |
CHEMBL1687696 |
| AMP-activated protein kinase, AMPK |
EC50 |
= |
5900.0 |
nM |
Activation of human AMPK after 15 mins |
CHEMBL1687696 |
| 6-phospho-1-fructokinase |
Potency |
|
31622.8 |
nM |
PUBCHEM_BIOASSAY: qHTS Assay to Find Inhibitors of T. brucei phosphofructokinase: hit validation. (Class of assay: confirmatory) [Related pubchem assays (depositor defined):AID485367, AID485368, AID488768] |
CHEMBL1201862 |
| Histone-lysine N-methyltransferase, H3 lysine-9 specific 3 |
Potency |
|
223.9 |
nM |
PUBCHEM_BIOASSAY: qHTS Assay for Inhibitors of Histone Lysine Methyltransferase G9a. (Class of assay: confirmatory) [Related pubchem assays (depositor defined):AID504404] |
CHEMBL1201862 |
| No relevant target |
LogP |
= |
-1.68 |
|
Octanol-water partition coefficient, log P of the compound |
CHEMBL1795329 |
| Adenosine deaminase-like protein |
Km |
= |
32800.0 |
nM |
Activity of recombinant human ADAL1 expressed in Escherichia coli by UV-spectrophotometry |
CHEMBL1811779 |
| Adenosine deaminase-like protein |
Kcat/Km |
= |
0.0017 |
/s/microM |
Kcat/Km ratio of recombinant human ADAL1 expressed in Escherichia coli |
CHEMBL1811779 |
| Adenosine deaminase-like protein |
Kcat |
= |
0.055 |
/s |
Activity of recombinant human ADAL1 expressed in Escherichia coli by UV-spectrophotometry |
CHEMBL1811779 |
| Plasmodium falciparum |
Potency |
|
15101.4 |
nM |
PUBCHEM_BIOASSAY: Primary qHTS for delayed death inhibitors of the malarial parasite plastid, 96 hour incubation. (Class of assay: confirmatory) [Related pubchem assays (depositor defined):AID488745, AID488752, AID488774, AID504848, AID504850] |
CHEMBL1201862 |
| Regulator of G-protein signaling 4 |
Potency |
|
1888.8 |
nM |
PUBCHEM_BIOASSAY: Inhibitors of Regulator of G Protein Signaling (RGS) 4: qHTS. (Class of assay: confirmatory) [Related pubchem assays (depositor defined):AID504856] |
CHEMBL1201862 |
| L-lactate dehydrogenase A chain |
Kd |
= |
650000.0 |
nM |
Binding affinity to 6-His-tagged human LDHA (2 to 322 amino acid residue) expressed in Escherichia coli BL21 (DE3) cells by NMR analysis in presence of NADH |
CHEMBL2057123 |
| L-lactate dehydrogenase A chain |
Kd |
|
|
|
Binding affinity to 6-His-tagged human LDHA (2 to 322 amino acid residue) expressed in Escherichia coli BL21 (DE3) cells by SPR analysis |
CHEMBL2057123 |
| L-lactate dehydrogenase A chain |
IC50 |
> |
500000.0 |
nM |
Inhibition of full length human recombinant LDHA expressed in Escherichia coli BL21 (DE3) cells after 10 mins by spectrophotometric analysis |
CHEMBL2057123 |
| L-lactate dehydrogenase B chain |
IC50 |
|
|
|
Inhibition of human recombinant LDHB (2 to 334 amino acid residue) expressed in Escherichia coli BL21 (DE3) cells after 10 mins by spectrophotometric analysis |
CHEMBL2057123 |
| Unchecked |
IC50 |
= |
12300.0 |
nM |
Inhibition of human recombinant FBPase using fructose-1,6-biphosphate as substrate incubated for 3 mins prior to substrate addition by spectrophotometric analysis |
CHEMBL2086262 |
| Adenosine A1 receptor |
EC50 |
= |
500.0 |
nM |
Agonist activity at human A1AR expressed in HEK293T/17 cells assessed as inhibition of isoproterenol-induced cAMP accumulation incubated for 10 mins by luciferase reporter assay |
CHEMBL2163273 |
| Streptavidin |
Activity |
|
|
|
Binding affinity to Streptomyces avidinii streptavidin at 4 mM using dye labeled Streptavidin binding aptamer by fluorescence spectral analysis method |
CHEMBL2202973 |
| Tyrosyl-DNA phosphodiesterase 1 |
Potency |
|
29092.9 |
nM |
PubChem BioAssay. qHTS for Inhibitors of human tyrosyl-DNA phosphodiesterase 1 (TDP1): qHTS in cells in absence of CPT. (Class of assay: confirmatory) |
CHEMBL1201862 |
| Tyrosyl-DNA phosphodiesterase 1 |
Potency |
|
29092.9 |
nM |
PubChem BioAssay. qHTS for Inhibitors of human tyrosyl-DNA phosphodiesterase 1 (TDP1): qHTS in cells in presence of CPT. (Class of assay: confirmatory) |
CHEMBL1201862 |
| Solute carrier organic anion transporter family member 1B1 |
Inhibition |
= |
91.52 |
% |
Inhibition of sodium fluorescein uptake in OATP1B1-transfected CHO cells at an equimolar substrate-inhibitor concentration of 10 uM |
CHEMBL3039007 |
| Solute carrier organic anion transporter family member 1B3 |
Inhibition |
= |
90.72 |
% |
Inhibition of sodium fluorescein uptake in OATP1B3-transfected CHO cells at an equimolar substrate-inhibitor concentration of 10 uM |
CHEMBL3039007 |
| Transient receptor potential cation channel subfamily M member 2 |
IC50 |
= |
70000.0 |
nM |
Inhibition of TRPM2 (unknown origin) |
CHEMBL3108672 |
| Transient receptor potential cation channel subfamily M member 2 |
Inhibition |
|
|
% |
Antagonist activity at human TRPM2 expressed in HEK293 cells assessed as inhibition of ADPR-induced maximum outward potassium current at 900 uM at +15 mV by whole-cell patch-clamp electrophysiology |
CHEMBL3108672 |
| Fructose-1,6-bisphosphatase |
IC50 |
= |
3200.0 |
nM |
Inhibition of human recombinant FBPase expressed in Escherichia coli BL21(DE3) by phosphoglucose isomerase and glucose-6-phosphate dehydrogenase coupled assay |
CHEMBL3124937 |
| Fructose-1,6-bisphosphatase |
IC50 |
= |
1000.0 |
nM |
Inhibition of FBPase (unknown origin) |
CHEMBL3217432 |
| NON-PROTEIN TARGET |
FC |
= |
0.12 |
|
Antiplatelet activity in sheep platelet rich plasma assessed as inhibition of ADP-induced platelet aggregation pretreated for 2.5 mins followed by ADP addition by photometric analysis relative to adenosine |
CHEMBL3244104 |
| NON-PROTEIN TARGET |
Inhibition |
|
|
% |
Antiplatelet activity in sheep platelet rich plasma assessed as inhibition of ADP-induced platelet aggregation pretreated for 2.5 mins followed by ADP addition by photometric analysis |
CHEMBL3244104 |
| NON-PROTEIN TARGET |
Inhibition |
|
|
% |
Antiplatelet activity in sheep platelet rich plasma assessed as inhibition of serotonin-induced platelet aggregation pretreated for 1 min followed by serotonin addition by photometric analysis |
CHEMBL3244104 |
| Unchecked |
Km |
= |
600000.0 |
nM |
Activity at rabbit muscle AMP aminohydrolase by Lineweaver-Burk plot analysis |
CHEMBL3272181 |
| Unchecked |
Vmax |
= |
294.0 |
micromol/min |
Activity at rabbit muscle AMP aminohydrolase by Lineweaver-Burk plot analysis |
CHEMBL3272181 |
| Unchecked |
Activity |
= |
100.0 |
% |
Activity at rabbit muscle AMP aminohydrolase after 24 hrs by Lineweaver-Burk plot analysis relative to AMP |
CHEMBL3272181 |
| Unchecked |
IC50 |
= |
1300.0 |
nM |
Inhibition of human recombinant FBPase-mediated fructose-6-phosphate formation by coupled spectrophotometric assay |
CHEMBL3286289 |
| 2'-deoxynucleoside 5'-phosphate N-hydrolase 1 |
Ki |
= |
19200.0 |
nM |
Inhibition of human DNPH1 assessed as 2-deoxyribose 5-phosphate production by spectrophotometrically |
CHEMBL3352723 |
| 2'-deoxynucleoside 5'-phosphate N-hydrolase 1 |
Ki |
= |
40000.0 |
nM |
Inhibition of rat DNPH1 assessed as 2-deoxyribose 5-phosphate production by spectrophotometrically |
CHEMBL3352723 |
| Adenylate kinase 1 |
Kcat/Km |
= |
2.85 |
10'6/M/s |
Ratio of Kcat to Km for recombinant AK1 (unknown origin) |
CHEMBL3352481 |
| Adenylate kinase 2 |
Kcat |
= |
80.0 |
/s |
Activity of recombinant AK2 (unknown origin) assessed as ADP formation by spectroscopic pyruvate kinase/lactate dehydrogenase coupled assay |
CHEMBL3352481 |
| Adenylate kinase 1 |
Kcat |
= |
500.0 |
/s |
Activity of recombinant AK1 (unknown origin) assessed as ADP formation by spectroscopic pyruvate kinase/lactate dehydrogenase coupled assay |
CHEMBL3352481 |
| Adenylate kinase 2 |
Kcat/Km |
= |
0.21 |
10'6/M/s |
Ratio of Kcat to Km for recombinant AK2 (unknown origin) |
CHEMBL3352481 |
| Adenylate kinase 1 |
Km |
= |
175000.0 |
nM |
Activity of recombinant AK1 (unknown origin) assessed as ADP formation by spectroscopic pyruvate kinase/lactate dehydrogenase coupled assay |
CHEMBL3352481 |
| Adenylate kinase 2 |
Km |
= |
380000.0 |
nM |
Activity of recombinant AK2 (unknown origin) assessed as ADP formation by spectroscopic pyruvate kinase/lactate dehydrogenase coupled assay |
CHEMBL3352481 |
| Fructose-1,6-bisphosphatase |
IC50 |
= |
3200.0 |
nM |
Inhibition of human liver FBPase expressed in Escherichia coli BL21(DE3) Rosetta cells assessed as reduction of NADP+ to NADPH by phosphoglucose isomerase and glucose-6-phosphate dehydrogenase coupling based spectrophotometry |
CHEMBL3392954 |
| Unchecked |
AC50 |
|
12589.3 |
nM |
PubChem BioAssay. qHTS Assay for Identifying Compounds that block Entry of Ebola Virus, Screen 2 green channel. (Class of assay: confirmatory) |
CHEMBL1201862 |
| Unchecked |
Ac50 |
|
10.0 |
uM |
PubChem BioAssay. qHTS Assay for Identifying Compounds that block Entry of Ebola Virus: Screen 2, blue channel. (Class of assay: confirmatory) |
CHEMBL1201862 |
| Unchecked |
Ac50 |
|
12.59 |
uM |
PubChem BioAssay. qHTS Assay for Identifying Compounds that block Entry of Ebola Virus: Screen 2, green channel. (Class of assay: confirmatory) |
CHEMBL1201862 |
| Unchecked |
AC50 |
|
10000.0 |
nM |
PubChem BioAssay. qHTS Assay for Identifying Compounds that block Entry of Ebola Virus, Screen 2 blue channel. (Class of assay: confirmatory) |
CHEMBL1201862 |
| Unchecked |
Activity |
|
|
|
Activity of Bacillus subtilis N-terminal His-tagged ANT(6) expressed in Escherichia coli BL21(DE3) cells assessed as adenylated product by Michaelis-Menten kinetics |
CHEMBL3779814 |
| No relevant target |
LogD |
|
|
|
Distribution coefficient, log D of the compound in phosphate buffer at pH 7.4 by shake flask method |
CHEMBL3853317 |
| No relevant target |
Solubility |
= |
432000.0 |
nM |
Apparent solubility of the compound in sodium phosphate buffer at pH 6.5 measured after 24 hrs by nitrogen detection method |
CHEMBL3853317 |
| NON-PROTEIN TARGET |
Papp |
|
|
|
Apparent permeability across apical to basolateral side in RRCK cells |
CHEMBL3853317 |
| AMP-activated protein kinase (AMPK) alpha-1/beta-1/gamma-1 |
EC50 |
= |
1519.0 |
nM |
Activation of recombinant human AMPK alpha1/beta1/gamma1 using Cy5-labelled SAMS as substrate assessed as protection from Thr172 residue dephosphorylation preincubated for 15 mins followed by incubation with PP2a for 60 mins measured 60 mins post okadaic acid/Cy5-labelled SAMS and ATP addition by TR-FRET assay |
CHEMBL3853317 |
| AMP-activated protein kinase (AMPK) alpha-1/beta-1/gamma-1 |
Activity |
= |
103.0 |
% |
Activation of recombinant human AMPK alpha1/beta1/gamma1 using Cy5-labelled SAMS as substrate assessed as protection from Thr172 residue dephosphorylation preincubated for 15 mins followed by incubation with PP2a for 60 mins measured 60 mins post okadaic acid/Cy5-labelled SAMS and ATP addition by TR-FRET assay relative to AMP |
CHEMBL3853317 |
| AMP-activated protein kinase (AMPK) alpha-1/beta-1/gamma-1 |
Kd |
= |
3700.0 |
nM |
Binding affinity to recombinant human AMPK alpha1/beta1/gamma1 by SPR binding assay |
CHEMBL3853317 |
| NON-PROTEIN TARGET |
CLH(app) |
|
|
|
Apparent intrinsic clearance in human hepatocytes measured after 4 hrs by LC-MS/MS analysis |
CHEMBL3853317 |
| NON-PROTEIN TARGET |
CLH(app) |
|
|
|
Apparent intrinsic clearance in rat hepatocytes measured after 4 hrs by LC-MS/MS analysis |
CHEMBL3853317 |
| NON-PROTEIN TARGET |
MTD |
= |
75.0 |
uM |
Cytotoxicity against African green monkey Vero cells incubated for 24 hrs by neutral red uptake assay |
CHEMBL3862035 |
| Mitogen-activated protein kinase kinase kinase 7 |
Kd |
= |
120000.0 |
nM |
Binding affinity to recombinant human biotinylated N-terminal GST-tagged autophosphorylated TAK1 (1 to 303 residues) fused with TAB1 (437 to 504 residues) expressed in baculovirus infected sf9 cells by SPR assay |
CHEMBL4002626 |
| Mitogen-activated protein kinase kinase kinase 7 |
Kd |
= |
370000.0 |
nM |
Binding affinity to recombinant human biotinylated N-terminal GST-tagged non-autophosphorylated TAK1 (1 to 303 residues) fused with TAB1 (437 to 504 residues) expressed in baculovirus infected sf9 cells by SPR assay |
CHEMBL4002626 |
| Adenosine kinase |
Drug metabolism |
|
|
|
Drug level assessed as human ADK-mediated compound formation treated with adenosine at 50 uM after 30 mins by HPLC analysis |
CHEMBL4007430 |
| Unchecked |
IC50 |
= |
1030000.0 |
nM |
Inhibition of BODIPY-FL-ATPgammaS binding to Thermotoga maritima His-tagged HK853 expressed in Escherichia coli BL21(DE3)pLysS Rosetta after 25 mins by fluorescence polarization assay |
CHEMBL4017529 |
| AMP-activated protein kinase (AMPK) alpha-1/beta-1/gamma-1 |
Activity |
|
|
|
Activation of human recombinant AMPKalpha1beta1gamma1 expressed in Escherichia coli at 10 uM incubated for 1 hr using serine/threonine kinase substrate |
CHEMBL4130473 |
| AMPK alpha1/beta1/gamma2 |
Activity |
|
|
|
Activation of human recombinant AMPKalpha1beta1gamma2 expressed in Escherichia coli at 10 uM incubated for 1 hr using serine/threonine kinase substrate |
CHEMBL4130473 |
| AMPK alpha1/beta2/gamma1 |
Activity |
|
|
|
Activation of human recombinant AMPKalpha1beta2gamma1 expressed in Escherichia coli at 10 uM incubated for 1 hr using serine/threonine kinase substrate |
CHEMBL4130473 |
| AMP-activated protein kinase alpha-1/beta-2/gamma-3 |
Activity |
|
|
|
Activation of human recombinant AMPKalpha1beta2gamma3 expressed in Escherichia coli at 10 uM incubated for 1 hr using serine/threonine kinase substrate |
CHEMBL4130473 |
| AMPK alpha2/beta1/gamma1 |
Activity |
|
|
|
Activation of human recombinant AMPKalpha2beta1gamma1 expressed in Escherichia coli at 10 uM incubated for 1 hr using serine/threonine kinase substrate |
CHEMBL4130473 |
| AMP-activated protein kinase alpha-2/beta-1/gamma-3 |
Activity |
|
|
|
Activation of human recombinant AMPKalpha2beta1gamma3 expressed in Escherichia coli at 10 uM incubated for 1 hr using serine/threonine kinase substrate |
CHEMBL4130473 |
| AMPK alpha2/beta2/gamma1 |
Activity |
|
|
|
Activation of human recombinant AMPKalpha2beta2gamma1 expressed in Escherichia coli at 10 uM incubated for 1 hr using serine/threonine kinase substrate |
CHEMBL4130473 |
| AMP-activated protein kinase alpha-2/beta-2/gamma-2 |
Activity |
|
|
|
Activation of human recombinant AMPKalpha2beta2gamma2 expressed in Escherichia coli at 10 uM incubated for 1 hr using serine/threonine kinase substrate |
CHEMBL4130473 |
| Transient receptor potential cation channel subfamily M member 2 |
Inhibition |
|
|
% |
Inhibition of human TRPM2 expressed in HEK293 cells assessed as reduction in ADPR-induced channel currents treated extracellularly after 60 secs by whole cell patch clamp electrophysiology method |
CHEMBL4152243 |
| Transient receptor potential cation channel subfamily M member 2 |
Inhibition |
|
|
% |
Inhibition of human TRPM2 D964A mutant expressed in HEK293 cells assessed as reduction in ADPR-induced channel currents at 30 uM treated extracellularly after 50 to 200 secs by whole cell patch clamp electrophysiology method relative to control |
CHEMBL4152243 |
| Transient receptor potential cation channel subfamily M member 2 |
Inhibition |
|
|
% |
Inhibition of human TRPM2 H995A mutant expressed in HEK293 cells assessed as reduction in ADPR-induced channel currents at 30 uM treated extracellularly after 50 to 200 secs by whole cell patch clamp electrophysiology method |
CHEMBL4152243 |
| Transient receptor potential cation channel subfamily M member 2 |
IC50 |
= |
70000.0 |
nM |
Inhibition of human TRPM2 assessed as reduction in ADPR-induced channel currents by whole cell patch clamp electrophysiology method |
CHEMBL4152243 |
| ADP-ribose glycohydrolase MACROD1 |
Inhibition |
|
|
% |
Inhibition of recombinant human N-terminal His6-tagged MDO1 (58 to 325 residues) expressed in Escherichia coli Rosetta2 (DE3) using mono-biotin-ADP-ribosylated SRPK2 as substrate after 16 hrs by Western blot analysis |
CHEMBL4219116 |
| ADP-ribose glycohydrolase MACROD1 |
Delta Tm |
< |
1.0 |
degrees C |
Binding affinity to human MDO1 assessed as change in melting temperature by DSF method |
CHEMBL4219116 |
| Fructose-1,6-bisphosphatase |
IC50 |
= |
2300.0 |
nM |
Inhibition of human FBPase expressed in Escherichia coli BL21 (DE3) using FBP as substrate incubated for 5 mins by malachite green dye based spectrophotometry |
CHEMBL4311987 |
| Replicase polyprotein 1ab |
Inhibition |
= |
11.47 |
% |
SARS-CoV-2 3CL-Pro protease inhibition percentage at 20µM by FRET kind of response from peptide substrate |
CHEMBL4495564 |
| SARS-CoV-2 |
Inhibition |
= |
-0.23 |
% |
Antiviral activity determined as inhibition of SARS-CoV-2 induced cytotoxicity of VERO-6 cells at 10 uM after 48 hours exposure to 0.01 MOI SARS CoV-2 virus by high content imaging |
CHEMBL4495565 |
| AMPK alpha2/beta1/gamma1 |
EC50 |
= |
1400.0 |
nM |
Allosteric activation of AMPK alpha2/beta1/gamma1 in human HepG2 cells by scintillation proximity assay |
CHEMBL4602612 |
| Fructose-1,6-bisphosphatase |
IC50 |
= |
2600.0 |
nM |
Inhibition of human liver FBP1 incubated for 5 mins by fluorescence method |
CHEMBL4627388 |
| SARS-CoV-2 |
Inhibition |
= |
-0.23 |
% |
Antiviral activity determined as inhibition of SARS-CoV-2 induced cytotoxicity of VERO-6 cells at 10 uM after 48 hours exposure to 0.01 MOI SARS CoV-2 virus by high content imaging |
CHEMBL4495565 |
| SARS-CoV-2 |
IC50 |
> |
20000.0 |
nM |
Antiviral activity determined as inhibition of SARS-CoV-2 induced cytotoxicity of VERO-6 cells at 10 uM after 48 hours exposure to 0.01 MOI SARS CoV-2 virus by high content imaging |
CHEMBL4651402 |
| SARS-CoV-2 |
IC50 |
< |
19952.62 |
nM |
Antiviral activity determined as inhibition of SARS-CoV-2 induced cytotoxicity of VERO-6 cells at 10 uM after 48 hours exposure to 0.01 MOI SARS CoV-2 virus by high content imaging |
CHEMBL4651402 |
| Fructose-1,6-bisphosphatase |
IC50 |
= |
2300.0 |
nM |
Inhibition of wild-type full-length human liver FBPase expressed in Escherichia coli BL21 (DE3) using FBP as substrate by malachite green dye based assay |
CHEMBL4706520 |
| Unchecked |
EC50 |
= |
1600.0 |
nM |
Activation of human recombinant AMPK alpha2/beta1/gamma1 using substrate-1 peptide as substrate incubated for 45 mins in presence of ATP by HTRF assay |
CHEMBL4715710 |
| Unchecked |
FC |
= |
3.2 |
|
Activation of human recombinant AMPK alpha2/beta1/gamma1 using substrate-1 peptide as substrate incubated for 45 mins in presence of ATP by HTRF assay relative to negative control |
CHEMBL4715710 |
| Fructose-1,6-bisphosphatase |
IC50 |
= |
3300.0 |
nM |
Inhibition of human liver FBPase expressed in Escherichia coli BL21(DE3) using FBP as substrate in presence of PGI and G6PDH by spectrophotometric method |
CHEMBL4725374 |
| Bis(5'-adenosyl)-triphosphatase |
Inhibition |
> |
50.0 |
% |
Inhibition of recombinant human FHIT at 100 uM incubated for 1 hr by fluorescence based analysis relative to control |
CHEMBL5032508 |
| Unchecked |
IC50 |
= |
3200.0 |
nM |
Cytotoxicity against paclitaxel-resistant human HCT116tax cells assessed as DNA damage by measuring fluorescence intensity incubated for 30 mins by Hoechst 33342 staining accumulation assay |
CHEMBL5104221 |
| HCT-116 |
Activity |
|
|
|
Cytotoxicity against human HCT-116 cells overexpressing human MDR1 assessed as DNA damage by measuring fluorescence intensity at 100 uM incubated for 30 mins by Hoechst 33342 staining accumulation assay |
CHEMBL5104221 |
| HCT-116 |
Activity |
|
|
|
Cytotoxicity against in human HCT-116 cells overexpressing human BCRP assessed as DNA damage by measuring fluorescence intensity at 100 uM incubated for 30 mins by Hoechst 33342 staining accumulation assay |
CHEMBL5104221 |
| HCT-116 |
EC50 |
> |
100000.0 |
nM |
Cytotoxicity against human HCT-116 cells incubated for 48 hrs by MTT assay |
CHEMBL5104221 |
| Unchecked |
EC50 |
> |
100000.0 |
nM |
Cytotoxicity against paclitaxel-resistant human HCT116tax cells incubated for 48 hrs by MTT assay |
CHEMBL5104221 |
| HCT-116 |
EC50 |
> |
100000.0 |
nM |
Cytotoxicity against human HCT-116 cells overexpressing human MDR1 incubated for 48 hrs by MTT assay |
CHEMBL5104221 |
| HCT-116 |
EC50 |
> |
100000.0 |
nM |
Cytotoxicity against human HCT-116 cells overexpressing human BCRP incubated for 48 hrs by MTT assay |
CHEMBL5104221 |
| HEK293 |
EC50 |
> |
100000.0 |
nM |
Cytotoxicity against human HEK293 cells incubated for 48 hrs by MTT assay |
CHEMBL5104221 |
| HCT-116 |
Activity |
|
|
|
Cytotoxicity against human HCT-116 cells assessed as reduction on cell viability at 100 uM incubated for 48 hrs in presence of paclitaxel by MTT assay |
CHEMBL5104221 |
| HCT-116 |
Activity |
|
|
|
Cytotoxicity against human HCT-116 cells assessed as reduction on cell viability at 100 uM incubated for 48 hrs by MTT assay |
CHEMBL5104221 |
| HCT-116 |
Activity |
|
|
|
Cytotoxicity against human wild type HCT-116 cells assessed as reduction on cell viability at 100 to 200 uM incubated for 48 hrs in presence of paclitaxel by MTT assay |
CHEMBL5104221 |
| Unchecked |
Activity |
|
|
|
Cytotoxicity against paclitaxel-resistant human HCT116tax cells assessed as reduction on cell viability at 100 to 200 uM incubated for 48 hrs in presence of paclitaxel by MTT assay |
CHEMBL5104221 |
| P-glycoprotein 1 |
Inhibition |
|
|
% |
Inhibition of recombinant human P-gp ATPase activity in presence of MgATP preincubated for 40 min measured after 20 min by Pgp-Glo assay |
CHEMBL5104221 |
| Unchecked |
IC50 |
= |
3300.0 |
nM |
Inhibition of recombinant human FBP expressed in expressed Escherichia coli BL21 (DE3) incubated for 1 hr by hanging drop vapour method |
CHEMBL5113382 |
